FastQCFastQC Report
Thu 5 Sep 2024
RNA-POC-57-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-57-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42513488
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCAC793060.186543150729011No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT666580.1567925925061712No Hit
TCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGTGTTA642070.1510273633628932No Hit
GTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAA632450.14876455208756334No Hit
ATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCT571890.13451966114848068No Hit
TGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCG560160.13176053679716893No Hit
GTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTTAACTC509690.11988901028304241No Hit
TATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTT508180.1195338288874345No Hit
TAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTA478740.11260896777041678No Hit
ATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACC465440.10948054885545971No Hit
TTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAA435130.10235104680189966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)