FastQCFastQC Report
Thu 5 Sep 2024
RNA-POC-47-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POC-47-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54219233
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACGTTCGAGTTGGCTATCGGTAGTGCAACGGGGGCTCTTGTCCCCCA1080320.19925032875326731No Hit
AACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCAC899610.1659208273934823No Hit
TAAGTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGG876270.16161608188002216No Hit
GTTCGCCGTTCGTCGCCTCCCGATCTGACCCGAGGGTCACAGCTGGGCCA694010.1280007041043904No Hit
GTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAA676660.12480073261087998No Hit
ATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCT595340.10980236478077808No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT560940.10345775271295335No Hit
TGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCG549930.1014271079784548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)