FastQCFastQC Report
Fri 7 Feb 2025
POC-47-TP2_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-47-TP2_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154898918
Total Bases23.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC6145560.396746476950859Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT5210900.3364064815481797Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC4121680.26608836609175024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT4044600.2611122177109074Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC3490340.22533017306163497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT3272470.21126487145636486Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC2571690.16602375492384008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC2510210.16205471493351556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC2431730.15698818503044676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGAGAGATCGGAAGAGCACACGTCTGAACTC2113780.13646189575062107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTAGATCGGAAGAGCACACGTCTGAACTCC1897060.1224708361100366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAG1867430.12055797575035354Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGAAGATCGGAAGAGCACACGTCTGAACTCC1850730.1194798533066577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAGATCGGAAGAGCACACGTCTGAACTCCA1587910.10251265925563147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)