FastQCFastQC Report
Thu 6 Feb 2025
401_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename401_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144497621
Total Bases21.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC7446520.5153385881695589Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT5629790.389611258721Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC5367260.3714427935114586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT5122250.3544868050111358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT4650460.3218364404767605Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC4552260.31504048083947345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC3724120.2577288106355744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC3178500.21996901942074187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAGAGATCGGAAGAGCACACGTCTGAACTC2732580.18910899578062948Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC2707720.1873885522309049Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTAGATCGGAAGAGCACACGTCTGAACTCC2702700.18704114166696212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAAGATCGGAAGAGCACACGTCTGAACTCC2351330.16272447834971623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAG2055330.14223971202958421Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGAGATCGGAAGAGCACACGTCTGAACTCCA1856350.1284692431026252Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAAGGAGATCGGAAGAGCACACGTCTGAACTC1727150.11952791942505406Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)