FastQCFastQC Report
Thu 6 Feb 2025
393_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename393_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171672115
Total Bases25.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC12139590.7071381394701172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT11541960.6723258462796944Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC9140130.5324178594758968Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT7326750.4267874255524841Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC6808850.39661945098072565Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT6645300.3870925688775955Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC5909400.34422596820689255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC5136550.29920700866299693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC4735860.2758665843896663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGTAGATCGGAAGAGCACACGTCTGAACTCC4275250.24903578545647903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAGAGATCGGAAGAGCACACGTCTGAACTC4161300.242398132043751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGAAGATCGGAAGAGCACACGTCTGAACTCC4083490.23786565453568276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAG4037080.23516224519049Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGAGATCGGAAGAGCACACGTCTGAACTCCA3084980.1797018694620265Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAGCAGATCGGAAGAGCACACGTCTGAACTCC2776410.16172748847417648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTACGGAGATCGGAAGAGCACACGTCTGAACTC2528520.14728775258579413Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTACAGATCGGAAGAGCACACGTCTGAACTCCA2451180.14278265284959063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAGACAGATCGGAAGAGCACACGTCTGAACTC2357800.13734321383528128Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGGAGATCGGAAGAGCACACGTCTGAACTC2260110.13165271482791482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGAAAGATCGGAAGAGCACACGTCTGAACTC2040810.11887836297700416Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAACAGATCGGAAGAGCACACGTCTGAACTCC1912250.11138966861333303Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATAGATCGGAAGAGCACACGTCTGAACTC1863360.10854179783362022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTAAGATCGGAAGAGCACACGTCTGAACTC1739840.10134668638526415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)