FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-79-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976568
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2753060.5508701597916847No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG2412950.4828162670153741No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2387450.4777138758307693No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2227290.4456668573160126No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG1687680.33769425703661765No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1401780.2804874476374608No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1334130.2669511039653623No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA1305760.2612744436552746No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG1250600.2502372711947727No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA1200260.24016455071504708No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG1188680.23784746483592073No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG1176220.23535429643748246No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1141470.22840103786238383No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1117030.22351074607604107No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT1116220.22334867012076537No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA1099940.22009114351349618No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG1086110.21732384664749285No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA1061850.2124695717401003No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA1051600.21041861057766112No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG1034670.20703102301862747No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT1016820.20345934918940412No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT979830.19605788056514806No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC934730.18703365145041573No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC907360.1815570849122733No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA905960.1812769536315499No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG894000.1788838321190843No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT882000.17648270685574088No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA852410.1705619321438799No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAG845810.16924131324904104No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC845790.16923731137360212No Hit
CGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATT834400.16695824331114534No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG819540.1639848498600384No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC805620.16119954455456006No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT805000.16107548641595398No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA784250.15692354064808933No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG782050.1564833343498097No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG774040.15488058323652798No Hit
TGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAACTTTA758680.1518071428994484No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG743840.14883775132378038No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA711470.1423607159259115No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG706740.14141427238461032No Hit
GTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTT703690.14080398638017722No Hit
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC692970.13865898114492375No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT655180.13109743750311145No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC650260.13011297614514064No Hit
TTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAA646420.12934461606087078No Hit
TTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATG641360.12833214157482764No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC639040.12786792402391456No Hit
GGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATAGCGAA637340.12752776461160759No Hit
GGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTG628890.12583697223866994No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA618690.12379601576482803No Hit
CCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAAATCCG617550.1235679088648104No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGTGTACA613830.12282356003317395No Hit
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAG595060.11906779993376096No Hit
GTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCA595020.11905979618288315No Hit
AGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGG588400.11773517541260536No Hit
GTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCT583130.11668068123445371No Hit
TGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCG563280.1127088198613398No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT555850.11122212313578635No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA555130.11107805561998574No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA549920.11003556706815083No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG548020.10965538890145479No Hit
ATCGTTGACCGACCTATTCTACTCTTAGGAAGGTTTGAGTAAGAGCGTGT539990.10804863591273414No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG539740.10799861246974783No Hit
GCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGCAGAAC536040.10725826551355028No Hit
AAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTA534090.10686808265825695No Hit
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGA530330.10611573007574271No Hit
TGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAAT529270.1059036306774807No Hit
CTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACAT518190.10368659168432694No Hit
ACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAAAGTCC517640.10357654010975705No Hit
GTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGAGCAGCA517330.103514511040454No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGA515620.10317235069042754No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC513640.1027761650219759No Hit
GATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTG509900.10202781431490053No Hit
GTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAA509170.10188174586138046No Hit
CCGAGGTGAGCAGCCTCTGGTTGATAGAACAATGTAGGTAAGGGAAGTCG508850.10181771585435799No Hit
CTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGA504270.1009012863788486No Hit
TTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGG503850.10081724699463157No Hit
CGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGAT503170.10068118322970876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)