FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-79-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976568
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG4726230.9456891877809617No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3289330.6581744468727825No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA3283050.656917857984966No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT3170120.6343212683191851No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA2411760.48257815542675925No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC2096220.4194405666271442No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1982120.3966098672481872No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1944020.38898629453707184No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1871880.374551529828939No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1803520.3608731195787594No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1749060.34997601275861917No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1628370.32582669542254283No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG1482730.2966850384764316No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1453480.290832295647032No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1434910.2871165543020081No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG1414510.28303464135432427No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC1392930.2787166177557451No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC1384630.2770558394485992No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1364140.2729559180614403No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA1358540.2718353929385467No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1347240.269574333315565No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT1322810.2646860424669417No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1265290.25317664870464895No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1194520.23901601246408116No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1072040.21450852727622274No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT1069510.21400229003320118No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT1031270.2063507041940135No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG1017890.20367344952538557No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC1005340.20116227268747225No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1001310.2003558947865328No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT983710.1968342444002958No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT956290.19134767317355605No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC955080.19110555970950224No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG927020.1854909284687176No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA883910.17686488596015637No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG878060.17569433739427645No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA874500.17498200356615123No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG874030.17488795949333696No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG872020.17448577101172694No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT867930.17366738748447072No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT856700.17142033442552518No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA837420.1675625265024201No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG821760.16442905803375693No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC820880.1642529755144451No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT815320.1631404541424293No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG793730.1588204296061306No Hit
ACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCT785920.15725769724723795No Hit
CAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCC780730.15621921057084193No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT760060.1520832723047329No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT750940.1502584171045919No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC744420.14895380571150865No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC744340.148937798209753No Hit
GACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCA742920.1486536650535907No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA741300.14832951314303935No Hit
GGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGAACG737220.1475131305535026No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC733140.14669674796396584No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT729400.14594839725689046No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC725230.14511400622787862No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT721720.14441167708835068No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG707710.14160836334339724No Hit
TGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCC706830.1414322808240854No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG698080.13968146031956416No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC687550.13757447290098032No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG685300.13712426191410343No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC682700.13660401810704567No Hit
TCCCTCTAAGAAGTTTACCACGAATCGAGATTCGCGTAACTATTTAGCAG674110.1348852126060357No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA667660.1335946077769886No Hit
AAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGCTACGA667640.13359060590154972No Hit
AGCTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAG666970.13345654307434637No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC657400.13154164567683No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG652760.13061321057500386No Hit
GGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTC649200.12990087674687867No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC647220.129504691078427No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC645800.1292205579222647No Hit
CGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTT643500.12876034224679053No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC643390.12873833193187656No Hit
ATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGA632720.12660333138522037No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA624880.12503459621316934No Hit
AGGTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAG619550.12396809640870099No Hit
TACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTA613730.12280355065597942No Hit
TCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCC611570.1223713481085776No Hit
CCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGTCGTCC607540.12156497020763811No Hit
GCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCACTTTA602630.12058250978738677No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT598590.1197741309487278No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA588830.11782121573454182No Hit
TTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTG580700.11619445336862666No Hit
TCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAG572690.11459170225534496No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC568870.11382734404651396No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG566060.1132650805473477No Hit
CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCT558070.1116663313095049No Hit
AGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAG555250.1111020668726192No Hit
CACCTCTAGCTTAAAACTCCTAAAGACTAAAGGATCGATAGGCCATGCTT554040.1108599534085654No Hit
ATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGAT552360.11052379587169732No Hit
CACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCA544340.10891904382069613No Hit
CTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCTA543220.10869493879611741No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT542900.10863090878909493No Hit
ACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTG537380.10752639116795694No Hit
CAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAA536180.10728627864162261No Hit
TTTTTAACTCTCTTTTCAAAGTTCTTTTCATCTTTCCCTCACGGTACTTG535280.10710619424687186No Hit
GGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGT533630.10677603952316214No Hit
CCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCAT532160.10648190167840256No Hit
ACTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATC530530.10615574883013175No Hit
TCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCA527130.10547543000551779No Hit
ATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTACA525940.10523731841690288No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA524360.10492117025722936No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC522800.1046090239729947No Hit
TGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATGA520070.10406276797558407No Hit
GCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAGC518370.10372260856327709No Hit
GAAGGACGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTC511730.10239398591756041No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA511620.10237197560264642No Hit
ATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC507810.1016096183315349No Hit
ACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACACGGA506990.10144554143853976No Hit
CCGCTTCAAGTCTCAGCCCGAGAGACCCAACGCTTAGAGCCAATCCTTTT506890.10142553206134523No Hit
TCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTGAGAATAGGTT506660.10137951049379781No Hit
TCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACACACA506090.101265457043789No Hit
TCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTGTCACCCTCTATGACGT501780.10040305288670481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)