FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-76-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-76-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49828147
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG3250990.6524404770661049No Hit
AAGAAATGTGTACAGAAGGCTGAAAGGAGAGAACTTTAGTCTTCTTGTTT2711220.5441141529906781No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2217320.44499346925343225No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2213450.44421679979389966No Hit
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2139070.42928949374737935No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1411430.28325958017262814No Hit
CCTTTTAAACCGATGCAATCTATTGGTTTAAAGACTAGCTACCAGGTGCA1359830.2729039873788604No Hit
CTATTGGAATCCTGGAGTGACCCCTGGCCTTCTCCCCGCTCCAACACCCT1319110.2647318994222282No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG1202010.24123112585342577No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1174830.23577637755624348No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1157470.23229240292640224No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1034130.20753932511277212No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC1009430.2025822874769957No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC947440.19014152783967664No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA932920.18722751219305828No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT912130.18305517160812743No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG861190.17283203407102415No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG858840.1723604130813855No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG856860.1719630473113921No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA814720.16350597986314844No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG793960.15933965997170235No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA769520.1544348016794604No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG764190.1533651251370034No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA757320.15198638632899594No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC739970.14850441859698293No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA738600.14822947359451277No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG724650.14542985112410461No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG716020.14369789829832524No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG715730.14363969826130601No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA714220.1433366566892403No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA698760.1402339926467665No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA698200.14012160636838453No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT693950.13926867479137844No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG688700.13821505343154744No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG674220.13530906537624207No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT673870.13523882395225334No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG669610.13438388547741903No Hit
ATTTCCACTGGGCGCCTCGGAGCTGCGGACTTCCCAGTGTGCATCGGGGC664560.133370402074153No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG655750.13160232508746514No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC639260.1282929505686816No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT629250.12628404584260378No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT610620.12254519518857485No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA600430.12050016630158852No Hit
CCGAGGTGAGCAGCCTCTGGTTGATAGAACAATGTAGGTAAGGGAAGTCG587850.11797548883365058No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT568240.11403996219245319No Hit
AGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCGCGGCT566540.11369878956165078No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTG563770.11314287886322563No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT557280.11184040217269166No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC548230.11002415963812583No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC547840.10994589062282409No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAAC524840.10533002561785008No Hit
ATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTTAACTC518070.10397135578812512No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA507740.10189823033154334No Hit
GGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATAGCGAA500870.1005194915235359No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA499970.10033887071899342No Hit
TCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA499820.10030876725156967No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAG498560.10005589812521022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)