FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-76-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-76-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49828147
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG3193340.6408707110862462No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA3063680.6148492738451623No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3050300.6121640445509643No Hit
ACAAAGTAAACATTGAAGGGAGATGGAAGAAGGAACTCTAGCCAGAGTCT2797340.5613975570875633No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2613510.5245047543108516No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1848780.3710312566911228No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1636700.32846896754960603No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1602560.32161741836396207No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1552380.3115468050618057No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1527900.3066339191782508No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA1477180.29645493339336904No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1397550.2804740059870177No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1378550.27666090011334354No Hit
CCGGTGCCTAATGAGTGAGCTAACTTACATTGACAGCTAGCTCAGTCCTA1370430.2750312990768049No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1337590.2684406466088334No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1334040.26772819787980473No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG1250370.2509364837508407No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1181030.23702065420975818No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1175160.23584260518457573No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1027890.20628702086794437No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1021290.20496246830129966No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT1015310.20376234340000643No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC986940.19806877426126243No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC966160.19389844057415984No Hit
GCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGT957870.19223472227454094No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT932000.1870428775928593No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC913690.18336824766933435No Hit
ATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGA878980.17640230530748013No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC857790.1721496888094193No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG833720.1673190857368226No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG830310.1666347335773895No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC805720.16169977181772383No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT779900.15651796162518347No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC777150.1559660647224148No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA771340.15480005708420183No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC768650.1542602015684027No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA752340.15098695121052766No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT751730.15086453044300444No Hit
TACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTA745750.1496644055417112No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT740580.14862683936450616No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT727520.14600583080081225No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG715180.1435293188807523No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG710020.1424937596013755No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC705290.14154449692861346No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC699430.14036845480125923No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA675410.13554788621780378No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT675240.13551376895472353No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG669570.134375857886106No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT664210.13330016065016426No Hit
TGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGA655980.13164848373751487No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC655370.13152606296999164No Hit
CCCCCACCGCCCCAAGGATCTCCCGGTCCCCGCCCGGCGTGCTGACGTCA654370.1313253731871667No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG643800.1292040821827069No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC627700.1259729766792251No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG627110.12585456970735837No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT623910.1252123624023185No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG623690.12516821065009703No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT615040.12343224402866115No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG609240.1222682432882764No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC608330.1220856155859057No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG600760.12056639392992077No Hit
ATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAATTTGAA590270.11846115810808698No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT574160.11522804570677694No Hit
TTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGACGGTG573230.11504140420874973No Hit
AGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAA554520.11128649837209478No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC546940.10976526981828162No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG535250.10741920625705788No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT526120.10558690853986602No Hit
TATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGC515430.10344153476146724No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA512270.10280735504774038No Hit
TCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTGAGAATAGGTT511540.10266085150627817No Hit
ACTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATC511340.10262071354971318No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA505300.10140854726145045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)