FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-73-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R2_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385213
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACATGG2476200.48188960508930845No Hit
TTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGC2309940.44953399336887057No Hit
ACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAA2144560.41734963714172013No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG2019530.39301773449883337No Hit
AGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCG1971540.3836784718592097No Hit
GGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCAC1801750.3506358920804707No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA1622690.31578929136675954No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG1326310.2581112196615785No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC1280350.24916701230760685No Hit
GCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAG1267880.24674024412431644No Hit
TTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAG1265210.2462206393890009No Hit
TTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATGGCCCG1240040.2413223430639472No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG1169360.22756741321671667No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA1162450.2262226683773793No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTG1125620.21905523676626582No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAA1117410.21745750085729915No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG1066770.207602525652662No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC1046500.20365781105159572No Hit
CGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATT1044460.2032608096807928No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGA1023300.199142893501288No Hit
TTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTG999770.19456375514099747No Hit
ATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCA995860.19380283584695854No Hit
CCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGA990850.19282784718631021No Hit
ATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGTTGGAT965190.1878341926888578No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG953990.18565457731974372No Hit
TGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTAT953760.18560981736127086No Hit
CTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGCCGAGG935680.1820912954082724No Hit
GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCT881000.17145010180263337No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA856110.16660629586180756No Hit
GGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGGCC830590.16163988655646908No Hit
GAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCGCAGAT803960.15645746179937017No Hit
GATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGAC796710.155046550064899No Hit
CAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACA796320.15497065274401023No Hit
AACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCT794740.15466317129015306No Hit
CTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGA786480.15305570495543144No Hit
ACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGA769740.1497979584126663No Hit
AAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAG749220.14580459168282517No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT739350.1438838056387934No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC732190.1424904086706812No Hit
GCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCG724750.14104252131834114No Hit
TGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAAT723910.14087905016565758No Hit
AAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAA712660.13868970437078854No Hit
TCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCA692410.13474888194002427No Hit
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC688800.13404634520051517No Hit
ATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC686210.13354230914640755No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT676720.13169547433811357No Hit
TTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATG669840.1303565677542292No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCAT669690.1303273764769643No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAA663450.1291130193427436No Hit
AAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGC660310.12850194860533126No Hit
TGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAACTTTA659090.12826452621690992No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG654260.1273245670889795No Hit
ATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTA631310.12285830166744661No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC609520.11861778212342916No Hit
GTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTT607810.11828500156260907No Hit
GTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTAC601140.11698696276689562No Hit
GTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCA597060.11619296002528977No Hit
GGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTG596530.11608981751228704No Hit
AAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCA593390.11547874677487471No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTG586260.11409118806221549No Hit
GTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGACAGCAG585810.11400361423042074No Hit
ACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAAAGTCC578210.11252458951566476No Hit
AATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGC571860.11128882544478311No Hit
AAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAG556020.1082062265656075No Hit
CCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAAATCCG555740.10815173618137966No Hit
TAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGT554230.10785787732357946No Hit
AGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAACGG552760.10757180280638323No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGTGTACA538930.10488036704255756No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA535980.10430627192301412No Hit
CTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGACAT535860.10428291890120218No Hit
TTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAA528860.1029206592955059No Hit
GTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATGGCGCT528350.10282140895280516No Hit
CTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGA525710.10230764247294256No Hit
AATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACTTTCTA519950.10118669742596961No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC518580.10092008376028333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)