FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-73-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-73-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385213
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG3930910.7649885580896589No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA3366940.6552351938290106No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2768080.5386919384765418No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA2690530.5236000481305779No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA2501250.48676454839255023No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT2275410.4428141613424858No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA2076780.40415907198827805No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1895240.3688298421571202No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1620830.31542731952867453No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1488020.28958136263831386No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1380920.2687387906711606No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1369350.26648717015145973No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1347520.2622388662668383No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA1331800.2591796204094746No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT1296830.25237416063644613No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1258540.2449226005932874No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA1239100.2411394110597537No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT1222760.2379595079230284No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG1214310.23631506597043783No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC1181430.22991633799396724No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC1122900.21852590160519525No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT1111850.21637547751334613No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG1098050.21368988000497344No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1069360.2081065617067696No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA1048540.20405481242239862No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC1048200.2039886455272648No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT1048090.20396723859060387No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG1022830.19905142749919125No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG1018810.1982691012684914No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC1000800.19476420191154992No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT985840.19185285852566183No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG971300.18902325071611553No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG964020.1876065007261914No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT935360.18202902068344057No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA877200.17071058944525538No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC875040.17029023505264052No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC869560.16922378038989544No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC845160.16447533262146835No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT805910.15683694840381415No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG805230.1567046146135465No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG799680.15562453735474444No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA771310.15010349378137247No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT769190.1496909237293616No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA767070.14927835367735073No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG766760.14921802503766987No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA757710.14745681797601967No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT756430.14720771907669236No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT756330.14718825822518242No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA752920.1465246431886932No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC752290.14640203982418054No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT744340.14485490212913976No Hit
CAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCC741960.14439173386320303No Hit
TCCCTCTAAGAAGTTTACCACGAATCGAGATTCGCGTAACTATTTAGCAG707410.13766800966651632No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA707320.13765049490015738No Hit
GGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACTCTTCG701990.1366132315146772No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC694020.1350622016493344No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT684740.13325623462921132No Hit
TCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGG681840.13269186993542287No Hit
ATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTT677320.13181223944717324No Hit
ACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCT672720.1309170402777157No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT669200.13023201830456554No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG668010.13000043417159718No Hit
TGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGG666260.12965986927017312No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC662020.12883472916615135No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA655610.12758728858436374No Hit
TCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCC649220.12634374017287814No Hit
GGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGT634620.12350245585242586No Hit
ATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGAT632810.12315021444009584No Hit
AAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGC622980.12123721273666803No Hit
GACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCA619950.12064754893591666No Hit
TCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAG618310.12032839097115351No Hit
ATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAATTTGAA614190.11952660388894369No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA608670.11845236488559463No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC602690.11728860596529977No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC602610.11727303728409182No Hit
ATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCAC601790.11711345830171026No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC588490.1145251650508873No Hit
CTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGAT585450.1139335551649849No Hit
TGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCC585160.11387711869560607No Hit
TTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGTAAATCCAAGAATTTCA580570.11298386561129951No Hit
TCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACACACA567440.11042865580804347No Hit
TTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGACGGTG559880.10895741543389145No Hit
AGCTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAG559230.10883091989907681No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC557560.1085059236788607No Hit
ACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCTGGCTT551910.10740638556854867No Hit
CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCT551650.10735578735462281No Hit
AAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGCTACGA550650.10716117883952335No Hit
CGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTT548350.10671357925479455No Hit
GTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGC547380.10652480899514807No Hit
TTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTG545850.10622705796704589No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG543550.10577945838231712No Hit
CTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCTA540590.10520341717762267No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA535000.10411555557821664No Hit
GGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGAACG533270.10377888284709455No Hit
CCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGTCGTCC533030.10373217680347067No Hit
ATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTACA529010.10294985057277081No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGAT524980.10216557825691994No Hit
GGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTC520440.10128205559836835No Hit
TGTAAAAGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGA519110.10102322627328604No Hit
CTTTCCGGGGCCCCCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACT517420.10069433788276795No Hit
TATTGTTATTTCTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGC516880.10058924928461424No Hit
AGGTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAG516590.10053281281523539No Hit
TCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTGTCACCCTCTATGACGT514100.1000482376126377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)