FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-71-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-71-S1-TP2_R1_001.fastp-trim.20230519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50831351
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length15-130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGG2889990.5685447943337174No Hit
GGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAA2612270.5139092211025436No Hit
GCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCT2283190.44916964729109793No Hit
GTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCA2019570.3973079527239007No Hit
TGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCT1847360.36342925451656793No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA1807640.35561517930145115No Hit
AGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCCCTGTA1745320.3433550290646416No Hit
ATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCAT1450870.2854281799435156No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1269350.24971793490202532No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC1224610.24091628019093964No Hit
TTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAA1132820.22285852681743595No Hit
TCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACAGA1078810.21223319443152316No Hit
CCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGA1059280.20839107738844084No Hit
TGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGG1044840.2055503108701557No Hit
CATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCA979560.19270784284289433No Hit
TTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTA979520.19269997368356392No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT972910.1913995951042104No Hit
AAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGT955140.18790372107166697No Hit
ACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCA934610.1838648750453239No Hit
CGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCCTC906250.17828564108004918No Hit
TCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTAGCG889980.17508486052239688No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC862280.1696354676860743No Hit
ACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCATCCAT859120.16901380409897035No Hit
TCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCCACGGG833330.16394016362067576No Hit
TTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGTTATG811370.15961999514826983No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG798200.15702907443872582No Hit
AGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGG774790.1524236489405918No Hit
GCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACCTC767940.1510760554052557No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT745130.14658866729707815No Hit
TAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAG742680.1461066812880893No Hit
AGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT742000.145972905579472No Hit
CGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATC733730.14434595688790566No Hit
TATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCT733140.1442298867877818No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG732580.1441197185571558No Hit
GCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCGCCC718940.14143633522547924No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC680240.13382292357328845No Hit
TGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGTA665760.1309742878956729No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT656080.12906995133770888No Hit
GAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTATGCAAAGTCCGT644870.12686461943535596No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA619530.12187950699952868No Hit
TTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGAGCACA600920.11821838062104625No Hit
GCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCGGGAAT596600.11736851141335984No Hit
GTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAG588840.11584189450325647No Hit
ACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCG581420.11438216544746174No Hit
AAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGATCCCTAGTTGGCAT575020.11312309995459298No Hit
AGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCC570240.11218273541460663No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC567900.11172238959377648No Hit
ATTAACCCGATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACA559780.11012495024969925No Hit
AGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGC551920.10857866044126979No Hit
TGCAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAA542700.10676481921560574No Hit
TTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGACA540820.10639496872707555No Hit
CTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGG540010.10623561825063434No Hit
CACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGTCCACC534550.10516147800203068No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT534530.10515754342236545No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT523670.1030210666641538No Hit
GACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCACTTCAT523510.10298959002683207No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC511680.10066228615485746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)