FastQCFastQC Report
Tue 20 Aug 2024
RNA-POR-79-S1-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNA-POR-79-S1-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50299323
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG4300960.8550731388571572No Hit
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA3214300.6390344458512891No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC3190230.6342490931736795No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC3148710.6259945089121777No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC2380370.47324096191115733No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA2072010.4119359618418721No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA1890810.3759116201226008No Hit
CTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAAT1880720.37390562890876283No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA1839980.36580611631691345No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT1804630.35877818872432937No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT1619000.3218731194453651No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC1461430.2905466540772328No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA1433470.2849879311496896No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC1424240.28315291639213513No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA1405110.2793496842889913No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG1379270.27421243820717034No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1366880.2717491843776903No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC1324760.26337531421645577No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA1320230.262474705673474No Hit
CCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAAT1300510.25855417576892636No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA1256090.2497230429920498No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG1152940.22921580872967218No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC1101170.2189234236810702No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA1075380.21379611809089358No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC1074500.21362116543795232No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA1030920.20495703292070155No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT1002740.19935457182992303No Hit
CTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC992540.19732671153446737No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA950020.18887331744007765No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA944350.18774606568760377No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT939780.1868375047513065No Hit
CCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGAC908310.18058095931032708No Hit
TTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATT905090.17994079164842836No Hit
CCGTGTTCCGGTTCGGGAATATTAACCCGATTCCCTTTCGATAGACGGCC882090.1753681654920087No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC879730.17489897428639348No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT878590.1746723310769014No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG864320.1718353147615923No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG809970.16103000034413983No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC809350.16090673824774937No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA793520.15775957859313533No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC787930.1566482316272925No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC779870.15504582437421674No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC769730.15302989266873432No Hit
CAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACC764010.15189270042461606No Hit
CCCTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGAC753870.14987676871913366No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG744020.1479184918651887No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC736970.14651688254332965No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT735980.14632006080877075No Hit
CCTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTG735570.14623854877728673No Hit
CTGCGGTTATGAGTACGACCGGACACGAAAATCATTGTCTCCCGCGGATT720250.14319278213744546No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA714420.14203372081170954No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA714300.142009863631763No Hit
CGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGT713080.14176731563563988No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG691840.13754459478510278No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC690120.13720264187253575No Hit
CTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCACGAATCGAGA679530.13509724574225382No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG678780.13494813836758798No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC678210.13483481676284192No Hit
CCCTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGT676660.1345266615218658No Hit
CGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACG674520.1341012084794859No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC670510.13330398104960578No Hit
CTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGC663290.1318685740561558No Hit
TGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATT660900.13139341855555392No Hit
ATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCC657010.13062004830562032No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC651510.12952659422473736No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG647980.1288247955146434No Hit
CCCCGTTCTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATT646990.12862797378008448No Hit
CATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTT638830.12700568554371994No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC636090.12646094660160734No Hit
CCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAG633490.12594404103609902No Hit
CTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCC633070.12586054090628615No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC632740.12579493366143318No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC611910.12165372484238007No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG610800.12143304592787461No Hit
GTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCTAGCACGGAT610360.12134556960140398No Hit
TCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTT608280.12093204514899733No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA606320.12054237787653722No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA601880.11965966221851534No Hit
CCCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGA599450.11917655432459796No Hit
CCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCAT594250.11814274319358135No Hit
CCCCAGCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGT584780.11626001407613379No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG584270.11615862106136102No Hit
CTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATT574050.11412678456924757No Hit
CCCCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACG572060.11373115300180084No Hit
CCACCGTCCTGCTGTCTATAGCAACCAACACCTTTTGTGGGGTCTGATGA567490.11282259206550356No Hit
GGTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGT567450.11281463967218804No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT567060.1127371038373618No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT564710.11226990073007544No Hit
CCCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAA559740.11128181586062301No Hit
CTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGC559460.11122614910741442No Hit
CCCTGTGGTAACTTTTCTGACACCTCTAGCTTAAAACTCCTAAAGACTAA551900.10972314677078257No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG547380.10882452632612967No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA547170.10878277626122325No Hit
TCCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATG543650.10808296564945816No Hit
CCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCT543150.10798356073301425No Hit
CCCCCGCCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCAC540450.10744677418421714No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA540230.10740303602098182No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC530560.1054805449369567No Hit
CTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTG530450.10545867585533904No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC528600.1050908776644966No Hit
CTCATGTCTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTC527830.10493779409317298No Hit
GCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGC522700.10391789965045851No Hit
GCCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGC519810.10334333923341275No Hit
CTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGTCGTCCGGCTTC519460.10327375579190201No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG518790.10314055320386718No Hit
GTTCCGGTTCGGGAATATTAACCCGATTCCCTTTCGATAGACGGCCCAAG509830.1013592171011924No Hit
CCAGCCCAGGCCGGCTCCACCCGCTTCAAGTCTCAGCCCGAGAGACCCAA507920.10097949032037669No Hit
CTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACA503650.10013057233394572No Hit
CTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAAT503110.1000232150241863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)