FastQCFastQC Report
Sat 8 Feb 2025
POR-76-TP2_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-76-TP2_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177234842
Total Bases26.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT5145700.29033230384802106Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC4145580.2339032186459139Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC4077180.23004393233244735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC3585120.20228076824758873Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAG3551220.20036805178521275Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT3273950.18472383663704228Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC2950150.16645429119405314Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC2631930.14849958226610996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTAGATCGGAAGAGCACACGTCTGAACTCC2534300.14299107169909628Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAAGATCGGAAGAGCACACGTCTGAACTCC2388860.13478501027467274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAAAGATCGGAAGAGCACACGTCTGAACTC2188890.12350224003923563Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGAGAGATCGGAAGAGCACACGTCTGAACTC2121200.11968301356908141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC2105410.11879210522274171Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2004370.11309119456319994Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGAGATCGGAAGAGCACACGTCTGAACTCCA1804980.10184114927018696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAGTAAGATCGGAAGAGCACACGTCTGAACTC1788090.10088817637787044Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)