FastQCFastQC Report
Sat 8 Feb 2025
POR-71-TP2_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-71-TP2_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239166304
Total Bases36.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC5958390.2491316669759633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT5377880.22485943504817468Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC4660810.19487736867815625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC3355090.1402827214321964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC3063340.12808409666271384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT3056450.1277960126021766Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2920090.12209454054196531Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC2510830.1049825982175148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC2401260.10040126722868119Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)