FastQCFastQC Report
Thu 6 Feb 2025
421_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename421_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272397195
Total Bases41.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21645410.7946267581793565No Hit
TGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT5857450.2150334183874397Illumina Single End PCR Primer 1 (100% over 31bp)
GTAGGGAAAGAGTGTATGGCCCAGAGGTATCCAGGAGATCGGAAGAGCGT4849710.17803817693497173No Hit
CCTAATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAG3172570.11646852677759768Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)