FastQCFastQC Report
Thu 6 Feb 2025
421_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename421_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272397195
Total Bases41.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGGAGATCGGAAGAGCACACGTCTGAACTCC20249170.7433692553258487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT18473200.6781714473968794Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGAAGATCGGAAGAGCACACGTCTGAACTC15796050.5798903325711559Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGATACGGCGACCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT12569510.4614405078583867Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGGGAGATCGGAAGAGCACACGTCTGAACTC11471770.4211412676257551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGTAGATCGGAAGAGCACACGTCTGAACTC10508290.3857708593511765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGGCAGATCGGAAGAGCACACGTCTGAACTC8237070.3023918803569178Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAGATCGGAAGAGCACACGTCTGAACTCC8037310.2950584715088568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAG7824630.2872507552803545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGTAGATCGGAAGAGCACACGTCTGAACTCC7811780.2867790176767422Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT7462840.2739690472950722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGAAGATCGGAAGAGCACACGTCTGAACTCC7312600.2684535719980523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGAGAGATCGGAAGAGCACACGTCTGAACTC6544370.240251005521551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGGATACCTCTGGGCCATACACTCTTTCCCTACAGATCGGAAGAGCAC4985960.18304006397716394No Hit
CGGTGGTCGCCGTATCATTAGAGATCGGAAGAGCACACGTCTGAACTCCA4956990.18197654348092682Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAGCAGATCGGAAGAGCACACGTCTGAACTCC4452380.16345175654250038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAAGGAGATCGGAAGAGCACACGTCTGAACTC4343690.1594616273489894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTACAGATCGGAAGAGCACACGTCTGAACTCCA4239830.15564881275668055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGGTCGCCGTATCATTAGACAGATCGGAAGAGCACACGTCTGAACTC4075290.14960836876459024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGAAAGATCGGAAGAGCACACGTCTGAACTC3865680.14191335560558913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTACGGAGATCGGAAGAGCACACGTCTGAACTC3645030.13381305192955456Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAACAGATCGGAAGAGCACACGTCTGAACTCC3376490.12395465379149738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGGTCGCCGTATCATTAGATAGATCGGAAGAGCACACGTCTGAACTC3356200.12320978562205827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTAAGATCGGAAGAGCACACGTCTGAACTC3347460.12288893062940681Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAAGAAGATCGGAAGAGCACACGTCTGAACTC3322010.12195463319657164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGGTCGCCGTATCATTAGTGAGATCGGAAGAGCACACGTCTGAACTC2846410.10449483519828463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGGATACCTCTGGGCCATAACACTCTTTCCCTACAGATCGGAAGAGCA2766320.10155464339491455No Hit
AATGATACGGCGACCAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT2763790.10146176431809438Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)