Bismark report for: ../data/22-Apul-meth/ACR-145-TP2_R1.fastp-trim.fq.gz and ../data/22-Apul-meth/ACR-145-TP2_R2.fastp-trim.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/github/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 10 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 243394673 Number of paired-end alignments with a unique best hit: 424134 Mapping efficiency: 0.2% Sequence pairs with no alignments under any condition: 242830578 Sequence pairs did not map uniquely: 139961 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 206828 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 217306 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 9757474 Total methylated C's in CpG context: 687306 Total methylated C's in CHG context: 523833 Total methylated C's in CHH context: 4263965 Total methylated C's in Unknown context: 55105 Total unmethylated C's in CpG context: 705848 Total unmethylated C's in CHG context: 705222 Total unmethylated C's in CHH context: 2871300 Total unmethylated C's in Unknown context: 105413 C methylated in CpG context: 49.3% C methylated in CHG context: 42.6% C methylated in CHH context: 59.8% C methylated in unknown context (CN or CHN): 34.3% Bismark completed in 0d 4h 55m 37s