Bismark report for: ../data/22-Apul-meth/ACR-140-TP2_R1.fastp-trim.fq.gz and ../data/22-Apul-meth/ACR-140-TP2_R2.fastp-trim.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/github/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 10 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 253164476 Number of paired-end alignments with a unique best hit: 473894 Mapping efficiency: 0.2% Sequence pairs with no alignments under any condition: 252531245 Sequence pairs did not map uniquely: 159337 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 231705 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 242189 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 10273551 Total methylated C's in CpG context: 765854 Total methylated C's in CHG context: 555949 Total methylated C's in CHH context: 4532164 Total methylated C's in Unknown context: 57277 Total unmethylated C's in CpG context: 734199 Total unmethylated C's in CHG context: 754999 Total unmethylated C's in CHH context: 2930386 Total unmethylated C's in Unknown context: 108119 C methylated in CpG context: 51.1% C methylated in CHG context: 42.4% C methylated in CHH context: 60.7% C methylated in unknown context (CN or CHN): 34.6% Bismark completed in 0d 4h 56m 28s