FastQCFastQC Report
Thu 6 Feb 2025
439_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename439_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214249351
Total Bases32.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7485300.349373287016398No Hit
CCTCAAAATAAACAACTTTTTCTACAAAATACCATACACTAAAATTTTAA2808340.13107811001023756No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)