FastQCFastQC Report
Wed 15 Nov 2023
M-T-8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525548
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAA1433180.44063208404667004No Hit
GAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTA1240760.3814724351454432No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA970920.2985099590020743No Hit
GAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATA951010.2923886170957058No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG888560.2731883256817072No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT700950.21550751427769949No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT591620.18189393765171918No Hit
TTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAG551270.1694883050087273No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC539050.16573125839417063No Hit
TTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAGA529840.16289963815521263No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG513870.1579896517039467No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC508780.1564247280322533No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT480900.14785300465959866No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT469150.14424045983790956No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA462860.1423065954184692No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA448700.13795309459505495No Hit
CCCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACA424780.1305988756899653No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT423960.13034676617900487No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG420120.12916615578621457No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA400310.12307555894215833No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC399150.12271891621933625No Hit
CCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACAC395740.12167050959448862No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC391050.12022856617204421No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTA385750.11859907786949508No Hit
TGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCT384240.11813482742858014No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG375150.11534010126439684No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTGTATACG371030.11407340469713224No Hit
CTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACACTTTTC360160.11073141642379092No Hit
CTCCCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAA352350.1083302270572044No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC339230.1042964748818375No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCA328380.10096063562095865No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC326960.10052405573612472No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG326940.10051790672366226No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATC325660.10012436992606549No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)