FastQCFastQC Report
Wed 15 Nov 2023
M-T-8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525548
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAA1496130.4599861007722299No Hit
GAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTA1281740.39407176168100233No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1017580.312855605076969No Hit
GAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATA984470.3026759149453839No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG863390.26544979349771447No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT683460.21013020287928735No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT599510.18431972306815553No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC594140.18266871322198783No Hit
TTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAG564080.1734267474909262No Hit
TTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAGA545980.16786189121240938No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC517730.15917641110919947No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG511450.1572456211959903No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT501140.1540758052715976No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA479880.14753940502401375No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT466770.1435087273548781No Hit
CCCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACA442780.13613298690616987No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA442440.13602845369430824No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT441650.13578556770204148No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC425030.13067573834574595No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA423260.13013155074281915No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG416080.1279240552687998No Hit
CCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACAC412500.12682338203802132No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTA403200.12396409124298229No Hit
TGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCT393640.12102486328593141No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC393250.12090495754291364No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG392610.12070818914411527No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTGTATACG377440.1160441631913473No Hit
CTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAACACTTTTC371260.1141441183404504No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC358820.1103194325888068No Hit
CTCCCCAACTAAACTTCAATTAAAAACTTAGCCAACAATTAAACTATTAA357920.11004272702799657No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCA340030.10454243538033547No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT333920.10266391207305715No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATC332930.10235953595616591No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG331530.10192910508379444No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC326760.10046256561150023No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)