FastQCFastQC Report
Wed 15 Nov 2023
M-T-500_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-500_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37240310
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA3048360.8185646145265709No Hit
AGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA1146450.30785189489561177No Hit
AAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT1014030.2722936516908694No Hit
GAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC892460.23964891806754562No Hit
GGGAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA770900.2070068697065089No Hit
CTAAAATAAAATATTTTATAAAACACTTTATTATTAAGTTTTTATAAGAG744600.1999446298916416No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAAAATTTTAAAGA732470.19668740673748417No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC646500.17360220685595798No Hit
ATTTTTTAAAGGAACTCGACAAATTTATCCTCCGCCTGTTTATCAAAAAC627970.1686264158381066No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG588850.15812166977127742No Hit
TGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT555750.14923345160123533No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA552190.14827749822705558No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC537800.14441340579603124No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA531790.14279956316153114No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT509170.1367254998682879No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG495710.1331111368299566No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCC492770.13232166971757217No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC474260.12735124922429486No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCCCTAACTAA468270.12574277711436882No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC464400.12470358060929139No Hit
GGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTAAATGAAAAGG446700.11995066636126284No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA446400.11987010849265219No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC425020.11412901772299962No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC415660.11161561222234723No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC408700.10974666967058008No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG398390.10697816425266063No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT390050.10473865550528445No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC380770.10224673210292826No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)