FastQCFastQC Report
Wed 15 Nov 2023
M-T-500_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-500_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37240310
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA3114530.836332995079794No Hit
AGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA1156880.3106526234609755No Hit
AAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT1019070.27364702388352835No Hit
GAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC900340.24176490474971882No Hit
GGGAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA773500.20770503790113454No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAAAATTTTAAAGA764960.20541182390801793No Hit
CTAAAATAAAATATTTTATAAAACACTTTATTATTAAGTTTTTATAAGAG758920.2037899254866568No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC681560.1830167364342563No Hit
ATTTTTTAAAGGAACTCGACAAATTTATCCTCCGCCTGTTTATCAAAAAC680330.18268644917295263No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG592660.15914475470263273No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA565100.1517441718396007No Hit
TGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT560850.15060293536761643No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA543940.1460621568402626No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC536660.14410728589531074No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT531540.14273243160435561No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCC495010.13292316846986504No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG491740.13204508770200893No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC488460.13116432167186579No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC481980.12942427170987567No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCCCTAACTAA457340.12280778543465401No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA445470.11962037909995916No Hit
GGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTAAATGAAAAGG442400.11879600357784348No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC428540.11507423004803129No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC428520.11506885952345723No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG414250.11123699023987715No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC399540.10728696941566813No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC383730.1030415697398867No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT382460.10270054142943494No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)