FastQCFastQC Report
Wed 15 Nov 2023
M-T-43_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-43_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50292908
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1912230.3802186184978606No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1728830.34375224435222557No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1329830.2644170028903479No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1141650.2270001965287034No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG1088150.21636251377629626No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1019130.20263890884973285No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT1004790.19978761220170443No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG986790.19620857875229644No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT930680.18505193614972512No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG871780.17334054336249555No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA839670.16695594535913494No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA780810.15525250597957072No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC778820.1548568239482195No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC717070.14257875086483368No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC710900.14135193773245325No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG695180.13822624851997026No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA690900.13737523389977768No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG677310.13467306364547463No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA664240.13207428769082113No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA658520.13093695039467593No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA647990.12884321582677224No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC633890.12603963962473597No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC631690.12560220220314164No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC607790.12085004112309433No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC600030.11930708003601621No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA587290.11677391969460187No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA576360.11460065105004467No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT573480.11402800569813938No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA565710.11248305625914491No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA558640.11107729145429411No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA547170.10879665180625467No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT546490.1086614438759437No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT538140.10700117002580166No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC537780.10692958935681349No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC525710.10452964859379377No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT521480.1036885757331829No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT519340.10326306842308661No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA511780.10175987437433524No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT504300.1002725871409146No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT503600.10013340250677094No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)