FastQCFastQC Report
Wed 15 Nov 2023
M-T-399_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-399_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39457912
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1683860.4267483793871303No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1451260.36779949227926706No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1109180.28110458556448703No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA972300.24641445801795087No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC752890.19080837323576574No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC679640.17224428905411923No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG674770.17101006256996062No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG611050.15486121009140066No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA591710.14995978499825333No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA586760.1487052837463878No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT559860.14188789310493674No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG533540.1352174945293608No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG526990.13355749792335692No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA493100.1249685994535139No Hit
TTTTTAAACAATTAAAAGTAAAACTCTACACCCGGATCCTTTCGTACAAT473680.12004689959265966No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA472520.11975291546090934No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA470060.11912946635392163No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC451170.11434208682912568No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT439100.11128313125134447No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA421150.10673398024710481No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA409590.10380427631345522No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC402930.1021164019018543No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA396590.10050962656108109No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)