FastQCFastQC Report
Wed 15 Nov 2023
M-T-245_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-245_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29909520
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1854380.6199965763409109No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1717290.5741616716015503No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA1479500.49465855687419924No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1329470.44449727043429654No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1294970.43296248151090355No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1103110.3688156814285218No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1023190.34209509213120104No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC874480.29237513674575855No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA786200.2628594507701895No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT728340.24351443954968183No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT728150.2434509146251762No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT704600.23557716740355578No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG679060.22703808018316576No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC676370.22613870098884903No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA675850.22596484330072833No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA670750.22425970059031372No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT657390.2197928953724433No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC629800.21056840765080817No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC582680.19481422637340887No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG579180.19364403039567335No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG560420.18737177995501098No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG556430.1860377565403925No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC554530.18540250729533606No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA546700.18278461172228777No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA537260.17962842599948112No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT519480.17368383043258467No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT512120.17122307546226084No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT510730.1707583404882459No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT508130.16988905204764235No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA492160.16454961497208914No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC472580.15800320433092874No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA464190.15519807740144276No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC453460.15161059087541356No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT450840.15073461560065154No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC450240.1505340105758969No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA444510.1486182325894899No Hit
CTGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATA444090.14847780907216163No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA437250.14619091178995852No Hit
AGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT406760.13599683311534255No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA393840.13167713824895885No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA390150.1304434173467177No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA388450.12987503644324616No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA379540.12689605182563946No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA371200.12410764198154968No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA366820.12264322530084067No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTT365790.1222988533416785No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG360300.12046331736517338No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT352380.11781533103841185No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT351230.11743083807429874No Hit
AAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAACTCAGAT347840.11629741968443492No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA347230.11609347124260101No Hit
CTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTACAACTTCTAGCT342020.11435155094431473No Hit
GAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAAGAA339170.1133986770767301No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT334250.11175371587374186No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC328240.10974432220911604No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA323820.10826653186009003No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA323720.10823309768929759No Hit
AAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAA323300.10809267417196933No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG320010.10699268995289794No Hit
AAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATGGGAA313550.1048328425197061No Hit
CCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATCCCTGCGGTAGCT309620.10351887960756308No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT306750.10255931890581994No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG304050.10165659629442399No Hit
GTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCT301020.10064354091941295No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)