FastQCFastQC Report
Wed 15 Nov 2023
M-T-235_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-235_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35966847
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1945030.5407841282278649No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1782220.4955174413815034No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA1422230.3954280451661498No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1392860.38726219176231935No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1324550.3682697012612754No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1241650.34522069727157345No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1054280.29312549971366686No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT950810.2643573399692222No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC804620.22371157527375143No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG784860.2182176269162543No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC777830.21626304913522168No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT755640.21009347858598781No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG733090.20382381586020037No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT731550.20339564377160999No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT721520.20060696451929746No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA720900.20043458354856628No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG706730.19649484426588743No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG689010.19156808490886065No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC668330.18581834543350437No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA660480.18363577991698854No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA657900.18291845265168782No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA655870.18235404398945507No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA633080.17601765314596524No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT631720.17563952714565165No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC623010.1732178525407023No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA607470.16889720691947227No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA607060.16878321305173066No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC604440.16805476443347953No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC591640.16449593148935185No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT569630.15837640702839478No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA564120.15684444065947734No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA536170.14907339528538602No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT536000.1490261295353468No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT530680.14754698959294374No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC516520.1436100306485025No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA516250.1435349615160873No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC515590.14335145919240572No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT510890.14204470022073382No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC495240.13769347087889022No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC492160.1368371267017095No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA489750.13616706518644794No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT479520.1333227791693834No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA475220.13212723372721552No Hit
CTGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATA471220.1310150984321756No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA468840.13035337793162685No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA446790.1242227321177194No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT444920.12370280886728827No Hit
AGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT440780.12255174883692196No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA435780.12116157971812208No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA421730.11725520449429443No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC416380.11576772353717857No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG412960.11481684785991943No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG407230.11322371404977478No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT404120.11235902885788127No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA393550.10942021134073832No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA392900.10923948935529434No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT386370.10742392848614171No Hit
ATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGGTTTAA385110.10707360586820414No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG384140.10680391305915694No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT381440.10605322173500502No Hit
AAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATGGGAA378800.10531921244027868No Hit
CCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATCCCTGCGGTAGCT377820.10504673929299391No Hit
CTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTACAACTTCTAGCT370970.10314220760023808No Hit
AAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAACTCAGAT368130.10235259154075975No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT365790.1017019923931614No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT365140.10152127040771741No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC363480.10105973426027587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)