FastQCFastQC Report
Wed 15 Nov 2023
M-T-20_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-20_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37411320
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1549910.4142890440647376No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1402160.37479565008665827No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1076410.28772307419251714No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA914530.24445274852638185No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG902010.241106167865769No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA767650.2051919044823866No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT719060.192203857014401No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG616220.1647148510130089No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC613740.16405195005148177No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA573390.15326644448792504No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG564540.15090085033086242No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT510960.1365789819765782No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA508220.13584658333360064No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC482460.12896096689451214No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT457480.12228384349977493No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA450100.12031117854168204No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC447230.11954403105797924No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC440200.11766492067106962No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG436650.11671601002049647No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA431950.11545970577889259No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT431500.11533942133022838No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC430860.11516835011435042No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT411910.1101030383317135No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC410590.10975020394896517No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA409630.10949359712514822No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC389890.10421711931041192No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)