FastQCFastQC Report
Wed 15 Nov 2023
M-T-193_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-T-193_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36747760
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1229810.33466257535153165No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1022660.2782917924793239No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG1007100.2740575207849404No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA756570.20588193674934202No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA756280.20580302037457524No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT713240.19409074185746286No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC625400.17018724406603286No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG580110.15786268333090234No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT557410.1516854360646744No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA551460.1500662897548041No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC525390.14297197978870005No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC514360.13997043629325978No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC503500.13701515412095866No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG503490.13701243286665638No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG475280.12933577447985944No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA474740.1291888267475351No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA463360.12609203935151422No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA457610.12452731812768997No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC448480.12204281294968729No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT448470.12204009169538496No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC440560.11988757954226326No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA437170.11896507433378253No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC431850.11751736704495731No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG404570.11009378530827457No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT379810.10335595965577221No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA375920.10229739173217632No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC373960.10176402588892493No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT369260.10048503636684249No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)