FastQCFastQC Report
Wed 15 Nov 2023
M-C-488_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-488_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33853156
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA3024100.8932992835291339No Hit
AGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA1223570.3614345439462129No Hit
AAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT1008830.29800175794540396No Hit
GAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC918100.2712007116854925No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG861220.25439873316390355No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC849790.25102238621415385No Hit
GGGAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA785060.23190156923626262No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA746660.22055846137358656No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAAAATTTTAAAGA737850.21795604522071738No Hit
CTAAAATAAAATATTTTATAAAACACTTTATTATTAAGTTTTTATAAGAG737060.21772268440791753No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT640260.18912860000408824No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA600770.17746351329843515No Hit
ATTTTTTAAAGGAACTCGACAAATTTATCCTCCGCCTGTTTATCAAAAAC598540.17680478594078497No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA589100.17401627192454375No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC579750.1712543433173557No Hit
TGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT570580.16854558552827392No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG548930.16215031768382243No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC539310.15930863284947494No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC539250.15929090924343953No Hit
GGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTAAATGAAAAGG515510.15227826912208717No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA511310.15103761669960697No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC500880.14795666318378115No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG496820.1467573658420503No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC484750.14319196709458934No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCC477670.14110058158240843No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC474020.14002239554858636No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC464240.13713344776481107No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT462960.1367553441693885No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA460170.13593119648874094No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC454020.13411452687010925No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCCCTAACTAA438860.12963636241182358No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTA432630.12779606131847795No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT428010.12643134365374972No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT418510.12362510603147311No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG417680.1233799294813163No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT395480.11682219524820671No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT391020.1155047405329063No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT389950.11518866955860776No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT387380.11442950843342345No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA373850.11043283527243368No Hit
GAAATGTTTATCGAAATGGTTGATAGCTGGTTGTAAGGGAAATATATTAT373700.1103885262573451No Hit
GAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAATA359370.10615553834921623No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG356690.1053638839463003No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA345400.10202889207729998No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC344520.10176894585544698No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA340340.10053420130164525No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)