FastQCFastQC Report
Wed 15 Nov 2023
M-C-488_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-488_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33853156
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA2888720.8533089204445222No Hit
AGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA1142780.3375697084195045No Hit
AAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT1011240.29871365612116046No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC898230.26533124415342546No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG853400.252088756510619No Hit
GAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC844090.2493386436407879No Hit
CTAAAATAAAATATTTTATAAAACACTTTATTATTAAGTTTTTATAAGAG778370.22992538716331204No Hit
GGGAGATGATGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA744720.21998539811177428No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA742160.21922919092092918No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAAAATTTTAAAGA739020.21830165553840827No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT656630.19396419051742178No Hit
ATTTTTTAAAGGAACTCGACAAATTTATCCTCCGCCTGTTTATCAAAAAC636320.18796474987442827No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA608170.17964942470947168No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA598080.17666890496117998No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC583330.1723118518108031No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG575250.16992507286469835No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC558170.1648797530132789No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC545290.1610750855843396No Hit
TGAAAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT536870.15858787287070075No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA516330.15252049173790475No Hit
GGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTAAATGAAAAGG513900.15180268569346977No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCC509460.15049113884684784No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG502990.14857994332936048No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC500950.1479773407241558No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC483260.1427518308780428No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC477940.14118033780956787No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC461290.1362620371347357No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAGCCCTAACTAA458700.1354969681408729No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC456230.13476734635908097No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT456020.13470531373795694No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAAGATAGTTAAGTTTCCTTTTA445930.13172479398966525No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA440570.13014148518383337No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT430240.12709007101139994No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT422140.12469738419661672No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT412070.12172277231700347No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG396360.11708214147005969No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT393500.11623731624903746No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT393020.11609552740075402No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT384930.11370579452031002No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC382980.11312977732415849No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA374340.11057757805505637No Hit
GAAATGTTTATCGAAATGGTTGATAGCTGGTTGTAAGGGAAATATATTAT374010.1104800982218615No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA372700.11009313282342124No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG366600.10829123287648572No Hit
GAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAATA359830.10629141932882122No Hit
TGTTTATCGAAATGGTTGATAGCTGGTTGTAAGGGAAATATATTATAGTA342790.1012579152147587No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA340530.1005903260540908No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)