FastQCFastQC Report
Wed 15 Nov 2023
M-C-363_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-363_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37249221
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA2100790.5639822642197001No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1858620.49896882407285775No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1390850.3733903589554262No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1102010.2958477977297834No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA1073950.2883147542870762No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG1025530.2753158247255694No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT839350.22533357140542618No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG776540.20847147380612338No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA760570.20418413582394113No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC752680.2020659707219112No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG705630.18943483408686587No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG640800.17203044326752498No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA635430.17058880238059207No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT598020.1605456393302829No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC594300.15954696072704447No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA583420.15662609427456214No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC577600.1550636454920762No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA564810.15163001663846876No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT553210.14851585755310157No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC542150.14554666794239804No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC531570.1427063400869511No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA525750.14114389130446514No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA512120.1374847543791587No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC505300.13565384360655489No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC498600.13385514827276523No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT497120.13345782452739077No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC492580.1322390070922557No Hit
GGAAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA482950.1296537181274207No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA480900.1291033710476791No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT474800.12746575290796014No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC463860.12452877873607075No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA456340.12250994457038444No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT449650.12071393385649595No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG448290.12034882554993566No Hit
AGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTAT445640.11963740127612334No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC437500.11745212067656395No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG432020.11598094897071808No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC427530.11477555463508889No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA426710.11455541580319224No Hit
GAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAAGAA420430.11286947450525207No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT411560.11048821665290666No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT399960.10737405756753945No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA399460.10723982657248055No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA397980.10684250282710611No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT393530.10564784697108162No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT386260.10369612830292477No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT383800.10303571180723484No Hit
CTATACTATAATATATTTTCCTTACAACCAGCTATCAACCATTTCGATAA378060.10149473998395833No Hit
AAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAA376590.10110010085848506No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG374970.10066519243449414No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)