FastQCFastQC Report
Wed 15 Nov 2023
M-C-358_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-358_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34184751
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAA953870.27903377151993886No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG876890.25651495896518306No Hit
GAGAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTA797580.23331455595508066No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC769480.22509451655798224No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT709150.2074462967420766No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG684990.2003788180291265No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG679130.1986646034075252No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA599800.17545834983557435No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT584870.1710909054156925No Hit
GAATTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATA583080.17056728013025457No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC566880.16582832503299497No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC537140.1571285395643221No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC528380.15456599347469285No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA524860.15353629458936238No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC516830.1511872940072022No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA492770.14414906810349445No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG480870.1406679838036556No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG476650.1394335152536287No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA447690.13096190169704613No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC440820.12895223370209716No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA434970.12724094436142008No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT434010.1269601173926936No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC427800.1251435179387441No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT420040.12287349994153828No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA413000.1208141021708773No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA408100.11938071451800247No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA405930.11874592855744363No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC402590.11776888472874937No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA387980.11349504929844305No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC386070.11293632064191428No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT384040.11234248861429473No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC382460.1118802942282657No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT370330.10833192846716948No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA366190.10712086216453647No Hit
ATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGGTTTAA365260.10684881103858268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357770.10465777562633116No Hit
TTTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAG357220.10449688517549828No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT355300.1039352312380453No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG354460.1036895076404096No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT354130.10359297336990987No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT345710.10112988683170457No Hit
TTTAAAGAATTATTTGATCTAAGTTCTTTTTGTATCATGATCTATAAAGA344780.10085783570575078No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)