FastQCFastQC Report
Wed 15 Nov 2023
M-C-337_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-337_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37007223
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1667700.4506417571510297No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1405030.37966372132272663No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA1388980.37532673013589807No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1341350.3624562696855152No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1165550.31495202976997216No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1024380.2768054225522407No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC862360.23302478005442342No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA854320.2308522311982177No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT748900.20236590030005766No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC738000.1994205293382862No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA732850.19802890911322907No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG702220.1897521464931319No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT693310.1873445083950233No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT685750.18530166394814332No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG682150.18432888087820046No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA632770.17098553976881758No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT625480.16901565405218327No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC625420.16899944100101755No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC625160.16892918444596614No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT601740.16260069014094897No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG601620.16256826403861754No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA595950.16103613070345754No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC594420.16062269789873182No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA591910.15994445192496612No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG586140.15838529683786323No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA578330.1562748980111261No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC556250.15030849518214323No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT532180.1438043594894975No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT530200.14326932880102894No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT512000.13835136994742891No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA508170.13731643684801748No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC505460.13658414737036606No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC464770.12558899650481745No Hit
CTGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATA462450.12496209185974318No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA460910.12454595687982316No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG456500.12335429761914316No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT447400.12089531819234317No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA442600.119598274099086No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA440570.119049732534646No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA432750.11693663153271457No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA425270.11491540448738885No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT424210.11462897391679455No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA413880.11183762694109742No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT394050.10647921353082884No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA393970.10645759612927454No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA390820.10560641094307455No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA383670.10367435567916025No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC380030.10269076390844024No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC376730.10179904609432595No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA370380.10008316484595453No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)