FastQCFastQC Report
Wed 15 Nov 2023
M-C-329_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-329_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35127753
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAA1950340.555213423414814No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTA1798390.5119570272542056No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC1317210.37497701603629474No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATA1315040.3743592708591409No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1140530.32468060225770773No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1073320.3055475822777506No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA1010390.2876329721402903No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG887350.2526065359204729No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA855180.243448534837967No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT854120.24314677912931124No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA851830.242494872928536No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG846940.2411028112159636No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT843420.24010075452306898No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA810660.23077479507442447No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC748820.21317048090152535No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA727930.20722361603943185No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC726970.20695032785046055No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA721610.205424468795371No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAGA686070.1953071122994972No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAG668270.19023989379565498No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC631360.1797325322801034No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG630920.1796072751934916No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC610010.1736547168274612No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC606810.17274375619755697No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCT601530.17124067115821498No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA598030.17024430796925724No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT594140.1691369214535299No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA592520.1686757476346409No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC591770.1684622412370071No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG591470.16837683867795358No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTA579150.16486964025282233No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA570670.1624555945835761No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT562130.16002446840251922No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATT543290.15466118769395812No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA540570.1538868711585395No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA527590.1501917871034905No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG521280.14839548661139812No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA512040.14576508779254965No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT507760.14454667795005277No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT505070.14378090167053953No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT504030.14348483946582066No Hit
CTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTATCCCA477060.1358071494069091No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA472580.1345318045250432No Hit
ATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGGTTTAA472470.13450049025339025No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA472230.13443216820614742No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT470280.13387705157229957No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC459920.13092781653298463No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT449460.12795011397398517No Hit
AGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTAT448830.12777076859997277No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG443130.12614811997795589No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT441460.12567271239922462No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT440720.1254620527535593No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA439110.12500372568663873No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT432650.12316472391501955No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA431420.12281457342290013No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC415500.1182825442891266No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC410980.11699581239938688No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT400400.11398394881676605No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT396430.11285378828529112No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA385960.10987323897432323No Hit
CTGCAACGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAG383510.1091757847420528No Hit
CCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTC378410.10772394123814295No Hit
CCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCT374990.10675035206493282No Hit
CCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATCCCTGCGGTAGCT371320.10570559409251142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362700.1032516938957069No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT357050.10164327903353226No Hit
AAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATGGGAA355630.10123904025401227No Hit
CTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTACAACTTCTAGCT354750.1009885260807886No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)