FastQCFastQC Report
Wed 15 Nov 2023
M-C-329_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-329_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35127753
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAA2285220.6505454533342909No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTA2105610.5994149412289479No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATA1587570.4519418022553279No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC1263550.35970134497358824No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1182490.3366255735173269No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1053720.2999679484195872No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1020970.29064483572291117No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA964210.27448667154998496No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG891500.2537879379873799No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA866040.24654010747570446No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA843130.2400181987159839No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT828150.23575376426724476No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT818350.23296394733816309No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG797090.22691175265323688No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA793030.2257559713540459No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAGA790040.22490479251547915No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAG775960.22089656574390054No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC730880.20806340787012478No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC703450.20025476722066454No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA703110.2001579776537372No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTA673030.1915949477326375No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCT670300.19081778444525044No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC630560.17950479212262738No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATT626560.17836609133524708No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA625710.17812411741792877No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC618960.17620255983922456No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG608240.1731508417290454No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC592970.1688038514732212No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG590210.1680181479299288No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT587060.16712142105986683No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA586300.16690506791026458No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC565420.1609610498001395No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA556140.15831926397341725No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA550270.1566482205679367No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA547660.15590521830417106No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT542880.15454447086325163No Hit
AGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTAT526880.1499896677137305No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT522320.148691548816117No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA516700.1470916742098477No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT505660.14394886003667812No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT502730.14311476170992207No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT495480.1410508665327953No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC492000.14006019684777446No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA489120.13924033228086066No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA488700.13912076869818574No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT483540.13765184468246516No Hit
ATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGGTTTAA475190.13527480678880882No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC467900.13319952460380827No Hit
TTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGATTATTG455280.12960692361962348No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG450860.1283486592495683No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA449230.1278846386787108No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT441300.12562716436772942No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT430270.12248719694652829No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA424370.12080761328514239No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC421330.11994220068673336No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA418090.11901985304895532No Hit
CTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTATCCCA417840.11894868424974407No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT400080.11389285275377563No Hit
CTGCAACGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAG399050.11359963730102521No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC383670.10922133277354804No Hit
CGCTTTTTAGCTTATCCCAAAAAACCTGAATAATAAAAATAAACTTTATT383230.10909607568693619No Hit
CCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTC377300.10740795176964492No Hit
CCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATCCCTGCGGTAGCT375560.10691261692713451No Hit
GAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAGAGAAGAA372480.1060358173208517No Hit
CCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCT371690.1058109239153441No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA369020.10505084113976776No Hit
AAAGAATTATTTGATCTGAGTTCTTTTTGTATTATGATCTATAAAGAGAA366610.10436477391537113No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT355500.1012020324784224No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)