FastQCFastQC Report
Wed 15 Nov 2023
M-C-306_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-306_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34922791
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGTTTAAGGTAATAAACCTAAGAAATACTAAAGATCCTCTTTGAGAG1609530.46088240770905164No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACCTA1475290.42244332647983374No Hit
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1431670.4099529158479917No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1407360.4029918456402869No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1343360.384665704410624No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA961630.2753588623543863No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC882350.2526573549061414No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA860860.24650378029636863No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT848160.24286718664610743No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC830940.23793630927150125No Hit
GTTTAAGGTAATAAACCTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT776720.22241063149849621No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA741590.21235129803915154No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG718590.20576534103474145No Hit
GGAGAGCTGAGTTTAAGGTAATAAACCTAAGAAATACTAAAGATCCTCTT717600.20548185853759512No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA714490.20459132261221616No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA671480.1922755830139693No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG655090.18758237278343534No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG652230.18676342334723475No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT642260.18390855415880134No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT621260.1778952890678182No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACC618080.17698470892546933No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC607480.17394944178430644No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC604110.172984455910182No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC587400.16819961497349967No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT555820.1591568096604879No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG524630.15022567927059438No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA521440.14931223566867838No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACCTAAGAAATACTAAAGATCCTCT504090.14434413331969945No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA497590.14248288460106182No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG460260.13179359003694752No Hit
CTGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATA451950.12941405513665846No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT437360.12523626762820875No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA432770.12392193968689387No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC432600.12387326087425257No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC428550.12271355974956297No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA427020.12227545043579134No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT425060.12171421236063293No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA422320.12092962443923796No Hit
CTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTACAACTTCTAGCT420480.12040274787888518No Hit
TGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCACGCCGGTCTGAA405840.11621064307259979No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT403770.11561790694220286No Hit
GGGCAATATAAACCCTACTTTATTAACAAAATTTTACTACTTTATATACG401000.11482472864210652No Hit
AAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATGGGAA400590.114707326799854No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA395780.11333000274806215No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA393880.11278594543030654No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA391580.11212734972986553No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC387170.11086456406075905No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA383370.10977644942524784No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA376520.10781497962176047No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA374470.10722797041049784No Hit
CGATAATTTGTCTCTTAATTAGAGAATGGTATGAAGGGTTAGACGTGGGA374390.10720506273396076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370130.10598522895836132No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA365550.10467376447661357No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTT362860.10390349385305431No Hit
GAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATC357120.1022598680615189No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)