FastQCFastQC Report
Wed 15 Nov 2023
M-C-226_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-226_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47981112
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1900940.3961850654899369No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA1706540.3556691224663572No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1520150.31682258635439714No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG1480300.3085172348652528No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC1441640.3004598976363866No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1281720.2671301157005282No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG1254160.26138618879862563No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1112950.23195585796344198No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC1005400.209540787633267No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC956880.1994284751049538No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC880680.1835472258333654No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG841870.17545862630278347No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA835750.1741831243927819No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT822760.1714758090642001No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA820940.17109649313671596No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC805250.1678264563772511No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC804320.1676326301066136No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC767800.16002130171555842No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG766230.15969408962426715No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC761870.1587853987210634No Hit
GTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAGAAGT752660.15686589339571788No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA752540.1568408835543453No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA745130.15529652584958847No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT739720.15416899883437465No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG734000.15297686306228167No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA728550.15184099943327697No Hit
GGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTT704180.14676191748119552No Hit
CCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCT669150.13946112795384985No Hit
TGAGTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAA651070.1356929785203811No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT649960.13546163748768472No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC647030.13485098052750422No Hit
GTTTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACT645420.13451543182242212No Hit
GCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTAC623970.13004492267707343No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC610420.1272208947554196No Hit
CGCCGGTCTGAACTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTA607970.1267102771607294No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA585250.1219750805275209No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT569750.11874464268356265No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT556960.11607901042393516No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT554640.11559548682406526No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT530620.11058935024265383No Hit
TGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAA530490.11056225624783353No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC529560.11036842997719602No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA523190.10904082423100157No Hit
GGGAGAGCTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCT502600.10474955228215636No Hit
CTCAGATCATGTAGAAAATTAAAAGACGAACAGTCTTACAACTTCTAGCT496400.10345737714457306No Hit
GTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAA495420.103253130106697No Hit
GAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAAGAA486290.10135029800893318No Hit
CCAACATCGAGGTCGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAA485910.10127110017792001No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT485780.10124400618309973No Hit
ATTTTTTAAAGGAATTCGACAAATTTATCCTTCGCCTGTTTATCAAAAAC485130.10110853620899825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)