FastQCFastQC Report
Wed 15 Nov 2023
M-C-218_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-218_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33910129
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1489990.439393787030418No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA1441090.42497331696968776No Hit
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1414490.4171290530920717No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1161700.34258200551227624No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA1139390.33600285035777955No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1029100.3034786449794986No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA860340.2537118039273752No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC833010.24565226513883215No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA684850.2019603051347873No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT680560.20069519641166805No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC665810.19634546362238847No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC651080.192001628775874No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC638360.18825053717725462No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT602730.17774335214118472No Hit
CTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTATCCCA600200.17699726238139643No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG570840.16833908240219314No Hit
ATTTAATATACTCACTGGGCAGGCCAGACTTTTTATTATTTAAATACTCA552600.1629601586003993No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC550170.16224355855443665No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC545590.16089292966122304No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG541810.15977821847861445No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC538160.15870184392397918No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC523970.1545172535321231No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT518050.15277146247364615No Hit
CCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTC517850.1527124830459949No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG479560.1414208716221634No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA468990.13830380887079494No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA433690.12789393989034958No Hit
CCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCT431510.12725106412895096No Hit
ATTTTTTAAAGGAATTCGACAAATTTATCCTTCGCCTGTTTATCAAAAAC430780.12703578921802391No Hit
CCCTACTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTA411520.12135607033520869No Hit
TAAAAACTCAGCCAACAATTAAACTATTAACACTTTTCATTTAACCATAT410730.12112310159598627No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA391060.11532247488648599No Hit
CTCGTCTTTTAATAAATTTAAACCTTTTCATTTAAAAGGAAACTTAACTG381460.11249146235922605No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA369910.10908540041236646No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT369380.10892910492909065No Hit
GAAAAAGTTGACTAATAGTATAATTATATTAAGAAATACTAATTATCTCT364610.10752244557960838No Hit
GTTTTATAAAATATTTTTTTTTAGTACCTTTCAGTATTTATTAATATTAT363840.10729537478315107No Hit
CGCTACCTTAGTACAACTCTCGTTGCAGCCATTTAATATACTCACTGGGC361860.10671147844940371No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT359100.10589756234781648No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT347500.10247675554404408No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT345620.10192234892412233No Hit
GAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAATA340700.10047145500390164No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA340230.1003328533489212No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)