FastQCFastQC Report
Wed 15 Nov 2023
M-C-218_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-218_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33910129
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1514110.4465067060051585No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA1444360.42593763061178563No Hit
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1443680.42573710055777136No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA1167480.3442865109713974No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1167100.34417445005886No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA1048630.30923798608964304No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC856130.2524702869753164No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA852390.2513673716782381No Hit
GGGAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAA689030.2031929751726984No Hit
TTTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAAT687680.20279486403605249No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC686320.202393803928024No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC666550.19656368750469808No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC627210.1849624340856975No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT601620.1774160163177203No Hit
CTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTATCCCA593710.1750833799541134No Hit
ATTTAATATACTCACTGGGCAGGCCAGACTTTTTATTATTTAAATACTCA575400.16968381335264163No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG573590.16915004953239782No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC558360.16465876611675528No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC556590.16413679818204172No Hit
TTTTAAACAATTAAAAGTAAAACTCTATACCCGGATCCTTTCGTACAATG548200.16166261119207184No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC534450.15760777554104852No Hit
CCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTC527690.1556142708864363No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAGAT517000.1524618204784771No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC514510.15172752660421904No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG487700.1438213343275692No Hit
GGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTA479240.1413265045379214No Hit
ATTTTTTAAAGGAATTCGACAAATTTATCCTTCGCCTGTTTATCAAAAAC443430.13076623801696538No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA438480.12930649718259699No Hit
CCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCT431870.1273572270987232No Hit
CCCTACTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTA427290.1260065982055096No Hit
TAAAAACTCAGCCAACAATTAAACTATTAACACTTTTCATTTAACCATAT402850.11879931214652709No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA391870.11556134156847353No Hit
GTTTTATAAAATATTTTTTTTTAGTACCTTTCAGTATTTATTAATATTAT381730.11257108458655525No Hit
CTCGTCTTTTAATAAATTTAAACCTTTTCATTTAAAAGGAAACTTAACTG376920.11115262935154273No Hit
AGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCA375700.1107928548428701No Hit
GAAAAAGTTGACTAATAGTATAATTATATTAAGAAATACTAATTATCTCT367500.10837469830916892No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT364290.10742807849536638No Hit
CTTTTTAAGAGATCTTATTGAGGGAAGAGTTTGCGACCTCGATGTTGGAT359420.1059919294320585No Hit
CGCTACCTTAGTACAACTCTCGTTGCAGCCATTTAATATACTCACTGGGC355470.10482708573594635No Hit
GTTAGACGTGGGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTA347090.10235584771735903No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT346050.10204915469357254No Hit
GAAATATATTATAGTATAGAGATAAAATTATAAACTATTTTTATTTAATA343350.10125293242028069No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)