FastQCFastQC Report
Wed 15 Nov 2023
M-C-216_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM-C-216_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34443939
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAA1638750.47577311061896843No Hit
GAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTA1157480.3360475118713919No Hit
GAAAAATTTTATATGGTATTTTCGAAGGGAGATGATGAGAATTTTAAAGA1127880.32745383737905237No Hit
CTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAG902530.2620286837692983No Hit
CTTACAACCAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATC830890.24122966888310884No Hit
GAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATA826570.23997545693017283No Hit
CAGCTATCAACCATTTCGATAAACATTTCACCTCTACCAATCCTAACTAA715060.20760111089501115No Hit
CCCGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACC699600.20311265793380948No Hit
TTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGA624210.18122491739402977No Hit
TTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAG621120.18032780745547136No Hit
CCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACA596440.17316254102064227No Hit
CTGAGTTTAAGGTAATAAACTTAAGAAATACTAAAGATCCTCTTTGAGAG592670.1720680088302328No Hit
CCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACAC562430.1632885251596805No Hit
CCCTCTTGTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTT549000.15938943568562236No Hit
CTCCCCAACTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAA530200.1539312910756229No Hit
CTAAACTTCAATTAAAAACTCAGCCAACAATTAAACTATTAACACTTTTC518890.1506476945043945No Hit
CTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTAGCTCAC511760.14857766412836815No Hit
GTAAAAACTTAATAATAAAGTGTTTTATAAAATATTTTTTTTTAGTACCT488410.14179853239201243No Hit
GGATTATTGTCTCTAAAAAATACAGTTAAGTTTCCTTTTAAATGAAAAGG487570.1415546578456082No Hit
CTATACTATAATATATTTCCCTTACAACCAGCTATCAACCATTTCGATAA477900.13874719729354998No Hit
CGAGAGTTGTACTAAGGTAGCGCGATAATTTGTCTCTTAATTAGAGAATG467020.13558844126393327No Hit
CCCACGTCTAACCCTTCATACCATTCTCTAATTAAGAGACAAATTATCGC460010.13355325010882174No Hit
GTCGAATTCCTTTAAAAAATATTTCTTCATTACTTATTTTAAATAGAAAT450790.13087643663519435No Hit
CTTCATACCATTCTCTAATTAAGAGACAAATTATCGCGCTACCTTAGTAC441520.1281851068195191No Hit
CTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCCC439970.1277351002160351No Hit
CTTTATTAACAAAATTTTACTACTTTATATACGCTTTTTAGCTTATCCCA432650.12560990774022682No Hit
CTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTTTAGTATTTCTT428260.12433537290842374No Hit
CAAAAACATGGCTTTTTGAGTATTTAAATAATAAAAAGTCTGGCCTGCCC419030.12165565616638677No Hit
TTTTAATTGAAGTTTAGTTGGGGAGAGCTGAGTTTAAGGTAATAAACTTA416920.12104306653196663No Hit
ATTTTTTAGAGACAATAATCCCACGTCTAACCCTTCATACCATTCTCTAA410550.11919368455506787No Hit
CAACAATTAAACTATTAACACTTTTCATTTAACCATATCTAGTTTTTTAT404700.11749527253546697No Hit
TGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCT400580.11629912595072242No Hit
CGCAAACTCTTCCCTCAATAAGATCTCTTAAAAAGAATTACGCTGTTATC398660.11574169841608417No Hit
GAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAAGAA387980.11264100775465896No Hit
AAAGAATTATTTGATCTGAGTTCTTTTTGTATCATGATCTATAAAGAGAA383110.11122711603919633No Hit
GCTACACCAAAAAGTTTTTCTAATCCAACATCGAGGTCGCAAACTCTTCC361240.1048776680274576No Hit
CGGATCCTTTCGTACAATGGGAATAAAAAATAAAGATAGAAACCAACCTA357370.10375410315295239No Hit
CGCTGTTATCCCTGCGGTAGCTTTTGCTAACTTCTCTCAAAGAGGATCTT356460.10348990572768114No Hit
CGAAGGGAGATGATGAGAATTTTAAAGAATTATTTGATCTGAGTTCTTTT345280.10024405164577722No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)