--- title: "fastqc and multiqc" output: html_document date: "2023-11-15" --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ### FastQC to evaluate the quality of our sequence data Self to do notes: - download the software (dowload the win/linux zip file for command line access) or find file path for Raven - ```{bash} #making a directory for our fastqc output to be saved to, within our more general 'output' directory mkdir ../output/fastQC-output/ ``` ```{bash} /home/shared/FastQC-0.12.1/fastqc ../data/raw/*fastq.gz -o ../output/fastQC-output/ ``` check output folder contents ```{bash} ls ../output/fastQC-output/ ``` # MultiQC move to fastqc directory ```{bash} eval "$(/opt/anaconda/anaconda3/bin/conda shell.bash hook)" conda activate which multiqc cd ../output/fastQC-output pwd multiqc . ```