Bismark report for: ../../data/CF01-CM01-Zygote_R1_001.fastp-trim.20220827.fq.gz and ../../data/CF01-CM01-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/ceasmallr/data/genome/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 32085370 Number of paired-end alignments with a unique best hit: 5263477 Mapping efficiency: 16.4% Sequence pairs with no alignments under any condition: 24901496 Sequence pairs did not map uniquely: 1920397 Sequence pairs which were discarded because genomic sequence could not be extracted: 13 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1421695 ((converted) top strand) GA/CT/CT: 1213974 (complementary to (converted) top strand) GA/CT/GA: 1208589 (complementary to (converted) bottom strand) CT/GA/GA: 1419206 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 147756270 Total methylated C's in CpG context: 3133060 Total methylated C's in CHG context: 761746 Total methylated C's in CHH context: 5081089 Total methylated C's in Unknown context: 323668 Total unmethylated C's in CpG context: 15103667 Total unmethylated C's in CHG context: 26035662 Total unmethylated C's in CHH context: 97641046 Total unmethylated C's in Unknown context: 1696327 C methylated in CpG context: 17.2% C methylated in CHG context: 2.8% C methylated in CHH context: 4.9% C methylated in Unknown context (CN or CHN): 16.0% Bismark completed in 0d 1h 21m 24s