A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2024-11-21, 10:35 PST based on data in:
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF01-CM01-Zygote-0-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF01-CM02-Larvae-1-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF02-CM02-Zygote-2-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF03-CM03-Zygote-3-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF03-CM04-Larvae-4-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF03-CM05-Larvae-5-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF04-CM04-Zygote-6-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF05-CM02-Larvae-7-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF05-CM05-Zygote-8-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF06-CM01-Zygote-9-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF06-CM02-Larvae-10-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF07-CM02-Zygote-11-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF08-CM03-Zygote-12-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF08-CM04-Larvae-13-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/CF08-CM05-Larvae-14-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF01-EM01-Zygote-15-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF02-EM02-Zygote-16-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF03-EM03-Zygote-17-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF03-EM04-Larvae-18-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF03-EM05-Larvae-19-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF04-EM04-Zygote-20-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF04-EM05-Larvae-21-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF05-EM01-Larvae-22-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF05-EM05-Zygote-23-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF05-EM06-Larvae-24-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF06-EM01-Larvae-25-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF06-EM02-Larvae-26-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF06-EM06-Larvae-27-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF07-EM01-Zygote-28-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF07-EM03-Larvae-29-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF08-EM03-Larvae-30-bismark_summary.txt
/gscratch/scrubbed/sr320/github/ceasmallr/output/04-bismark-align/EF08-EM04-Larvae-31-bismark_summary.txt
General Statistics
Sample Name | % Aligned |
---|---|
CF01-CM01-Zygote-0-bismark | 6.2% |
CF01-CM02-Larvae-1-bismark | 17.9% |
CF02-CM02-Zygote-2-bismark | 5.9% |
CF03-CM03-Zygote-3-bismark | 13.6% |
CF03-CM04-Larvae-4-bismark | 22.8% |
CF03-CM05-Larvae-5-bismark | 16.3% |
CF04-CM04-Zygote-6-bismark | 10.3% |
CF05-CM02-Larvae-7-bismark | 18.9% |
CF05-CM05-Zygote-8-bismark | 13.1% |
CF06-CM02-Larvae-10-bismark | 11.0% |
CF07-CM02-Zygote-11-bismark | 6.0% |
CF08-CM03-Zygote-12-bismark | 7.9% |
CF08-CM04-Larvae-13-bismark | 0.6% |
CF08-CM05-Larvae-14-bismark | 1.1% |
EF01-EM01-Zygote-15-bismark | 10.9% |
EF02-EM02-Zygote-16-bismark | 13.3% |
EF03-EM03-Zygote-17-bismark | 10.0% |
EF03-EM04-Larvae-18-bismark | 13.5% |
EF03-EM05-Larvae-19-bismark | 13.4% |
EF04-EM04-Zygote-20-bismark | 4.4% |
EF04-EM05-Larvae-21-bismark | 19.8% |
EF05-EM01-Larvae-22-bismark | 13.1% |
EF05-EM05-Zygote-23-bismark | 4.3% |
EF05-EM06-Larvae-24-bismark | 16.4% |
EF06-EM01-Larvae-25-bismark | 15.6% |
EF06-EM02-Larvae-26-bismark | 16.5% |
EF06-EM06-Larvae-27-bismark | 11.7% |
EF07-EM01-Zygote-28-bismark | 7.1% |
EF07-EM03-Larvae-29-bismark | 16.0% |
EF08-EM03-Larvae-30-bismark | 20.4% |
EF08-EM04-Larvae-31-bismark | 18.0% |
Bowtie 2 / HiSAT2
Results from both Bowtie 2 and HISAT2, tools for aligning reads against a reference genome.URL: http://bowtie-bio.sourceforge.net/bowtie2; https://ccb.jhu.edu/software/hisat2DOI: 10.1038/nmeth.1923; 10.1038/nmeth.3317; 10.1038/s41587-019-0201-4
Paired-end alignments
This plot shows the number of reads aligning to the reference in different ways.
There are 6 possible types of alignment:
- PE mapped uniquely: Pair has only one occurence in the reference genome.
- PE mapped discordantly uniquely: Pair has only one occurence but not in proper pair.
- PE one mate mapped uniquely: One read of a pair has one occurence.
- PE multimapped: Pair has multiple occurence.
- PE one mate multimapped: One read of a pair has multiple occurence.
- PE neither mate aligned: Pair has no occurence.