Bismark report for: ../../data/EF06-EM01-Larvae_R1_001.fastp-trim.20220827.fq.gz and ../../data/EF06-EM01-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/ceasmallr/data/genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 3403 Mapping efficiency: 34.0% Sequence pairs with no alignments under any condition: 5320 Sequence pairs did not map uniquely: 1277 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 816 ((converted) top strand) GA/CT/CT: 904 (complementary to (converted) top strand) GA/CT/GA: 902 (complementary to (converted) bottom strand) CT/GA/GA: 781 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 141554 Total methylated C's in CpG context: 3681 Total methylated C's in CHG context: 580 Total methylated C's in CHH context: 2287 Total methylated C's in Unknown context: 125 Total unmethylated C's in CpG context: 16685 Total unmethylated C's in CHG context: 29231 Total unmethylated C's in CHH context: 89090 Total unmethylated C's in Unknown context: 698 C methylated in CpG context: 18.1% C methylated in CHG context: 1.9% C methylated in CHH context: 2.5% C methylated in Unknown context (CN or CHN): 15.2% Bismark completed in 0d 0h 0m 16s