Bismark report for: ../../data/CF02-CM02-Zygote_R1_001.fastp-trim.20220827.fq.gz and ../../data/CF02-CM02-Zygote_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/ceasmallr/data/genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 1389 Mapping efficiency: 13.9% Sequence pairs with no alignments under any condition: 7984 Sequence pairs did not map uniquely: 627 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 445 ((converted) top strand) GA/CT/CT: 242 (complementary to (converted) top strand) GA/CT/GA: 264 (complementary to (converted) bottom strand) CT/GA/GA: 438 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 33169 Total methylated C's in CpG context: 884 Total methylated C's in CHG context: 330 Total methylated C's in CHH context: 1676 Total methylated C's in Unknown context: 65 Total unmethylated C's in CpG context: 2779 Total unmethylated C's in CHG context: 5741 Total unmethylated C's in CHH context: 21759 Total unmethylated C's in Unknown context: 237 C methylated in CpG context: 24.1% C methylated in CHG context: 5.4% C methylated in CHH context: 7.2% C methylated in Unknown context (CN or CHN): 21.5% Bismark completed in 0d 0h 0m 15s