Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF07-EM03-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF07-EM03-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 129603536 Number of paired-end alignments with a unique best hit: 42354596 Mapping efficiency: 32.7% Sequence pairs with no alignments under any condition: 71124593 Sequence pairs did not map uniquely: 16124347 Sequence pairs which were discarded because genomic sequence could not be extracted: 38 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10116262 ((converted) top strand) GA/CT/CT: 11026738 (complementary to (converted) top strand) GA/CT/GA: 11066736 (complementary to (converted) bottom strand) CT/GA/GA: 10144822 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1695965684 Total methylated C's in CpG context: 44052670 Total methylated C's in CHG context: 6342424 Total methylated C's in CHH context: 26467737 Total methylated C's in Unknown context: 1429257 Total unmethylated C's in CpG context: 201680564 Total unmethylated C's in CHG context: 353894882 Total unmethylated C's in CHH context: 1063527407 Total unmethylated C's in Unknown context: 7539972 C methylated in CpG context: 17.9% C methylated in CHG context: 1.8% C methylated in CHH context: 2.4% C methylated in unknown context (CN or CHN): 15.9% Bismark completed in 0d 9h 46m 29s