Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF06-EM01-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF06-EM01-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 142739602 Number of paired-end alignments with a unique best hit: 48203619 Mapping efficiency: 33.8% Sequence pairs with no alignments under any condition: 76956067 Sequence pairs did not map uniquely: 17579916 Sequence pairs which were discarded because genomic sequence could not be extracted: 73 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11340961 ((converted) top strand) GA/CT/CT: 12731817 (complementary to (converted) top strand) GA/CT/GA: 12767680 (complementary to (converted) bottom strand) CT/GA/GA: 11363088 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2005229648 Total methylated C's in CpG context: 50372264 Total methylated C's in CHG context: 7529440 Total methylated C's in CHH context: 29934165 Total methylated C's in Unknown context: 1886211 Total unmethylated C's in CpG context: 243293816 Total unmethylated C's in CHG context: 411143204 Total unmethylated C's in CHH context: 1262956759 Total unmethylated C's in Unknown context: 9335214 C methylated in CpG context: 17.2% C methylated in CHG context: 1.8% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 16.8% Bismark completed in 0d 6h 24m 53s