Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM06-Larvae_R1_001.fastp-trim.20220827.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/output/02.00-bismark-bowtie2-alignment/trimmed-fastqs/EF05-EM06-Larvae_R2_001.fastp-trim.20220827.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/ceasmallr/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 198770863 Number of paired-end alignments with a unique best hit: 67541240 Mapping efficiency: 34.0% Sequence pairs with no alignments under any condition: 110632680 Sequence pairs did not map uniquely: 20596943 Sequence pairs which were discarded because genomic sequence could not be extracted: 24 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 16116899 ((converted) top strand) GA/CT/CT: 17630936 (complementary to (converted) top strand) GA/CT/GA: 17635168 (complementary to (converted) bottom strand) CT/GA/GA: 16158213 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3040036603 Total methylated C's in CpG context: 84390281 Total methylated C's in CHG context: 29856687 Total methylated C's in CHH context: 118081782 Total methylated C's in Unknown context: 3603787 Total unmethylated C's in CpG context: 341384062 Total unmethylated C's in CHG context: 574268764 Total unmethylated C's in CHH context: 1892055027 Total unmethylated C's in Unknown context: 13751394 C methylated in CpG context: 19.8% C methylated in CHG context: 4.9% C methylated in CHH context: 5.9% C methylated in unknown context (CN or CHN): 20.8% Bismark completed in 0d 12h 25m 0s